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KMID : 1377020230200030447
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Tissue Engineering and Regenerative Medicine
2023 Volume.20 No. 3 p.447 ~ p.459
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Schisandrin B Improves the Hypothermic Preservation of Celsior Solution in Human Umbilical Cord Mesenchymal Stem Cells
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Ying Zhang
Peng Wang
Mei-xian Jin
Ying-qi Zhou
Liang Ye
Xiao-juan Zhu
Hui-fang Li
Ming Zhou
Yang Li
Shao Li
Kang-yan Liang
Yi Wang
Yi Gao
Ming-xin Pan
Shu-qin Zhou
Qing Peng
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Abstract
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Background : Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs.
Methods : hUCMSCs were isolated from Wharton’s jelly. Subsequently, hUCMSCs were exposed to cold storage (4 °C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated.
Results : SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2?related factor 2 signaling.
Conclusion : These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs.
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KEYWORD
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Schisandrin B, Stem cells, Low temperature preservation
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